Welcome to Nitta lab wiki page

Molecular biology protocol

• List of the lab materials

Cell culture protocol

• Culture media list for insect cell

Molecular biology protocol

• Making Competent cell (Rubidium chloride method)
• Competent cell list
• E.coli media recipe
• Bacteria culture reagent list (Antibiotics, IPTG, X-gal etc)
• BEVS media list

Biochemistry protocol

• Amylose resin purification
• Reagent list for biochemistry
• SDSPAGE

X-ray crystallography protocol

• Workstation tips

Electron microscopy protocol

• Carbon coating
• Serial EM を使った JEM1400 plus での Cryo-EM
• Workstation tips