LB media
| Name | amount (for 1 L) |
| Bacto Tryptone | 10 g |
| Bacto Yeast Extract | 5 g |
| NaCl | 5 g |
| 5N NaOH | 0.5 ml |
Super Broth
| Name | amount (for 1 L) |
| Bacto Tryptone | 32 g |
| Bacto Yeast Extract | 20 g |
| NaCl | 5 g |
| 5N NaOH | 1 ml |
LB media plate
| Name | amount (for 1 L) |
| Bacto Tryptone | 10 g |
| Bacto Yeast Extract | 5 g |
| NaCl | 5 g |
| 5N NaOH | 0.5 ml |
| Bacto Agar | 7.5 g (for 1.5 %) |
Bacmid transformation
Make super broth plate with antibiotics, X-gal, and IPTG.
|
Total volume
|
100 ml
|
200 ml
|
500 ml
|
|
Gentamycin (10 mg/ml)
|
100 (µl)
|
200 (µl)
|
500 (µl)
|
|
Kanamycin (25 mg/ml)
|
100 (µl)
|
200 (µl)
|
500 (µl)
|
|
Carbenicillin (50 mg/ml)
|
100 (µl)
|
200 (µl)
|
500 (µl)
|
|
Tetracyclin (10 mg/ml)
|
100 (µl)
|
200 (µl)
|
500 (µl)
|
|
X-gal (50 mg/ml)
|
100 (µl)
|
200 (µl)
|
500 (µl)
|
|
IPTG (1 M)
|
50 (µl)
|
100 (µl)
|
250 (µl)
|