Culture media list for insect cell

Preparation of SFX insect cell media from powder (GE)

  1. While stirring, add SFX-Insect powder medium to cell culture-grade water at 90% of final preparation volume (41.32 g/L). Mix until dissolved. If your water source is normally cool, it might be useful to adjust the water temperature. Using warmer room temperature water (22°C to 25°C) will improve dissolution time. Mix for 20 min or until dissolved
  2. Add 0.35 g/L sodium bicarbonate and 1.5 mL glycerol. Mix until dissolved.
  3. Adjust pH to 6.1 to 6.4, if necessary.
  4. Bring vessel to final volume with cell culture grade water. Allow solution to mix for 10 to 20 min.
  5. Check pH and osmolality. Expected values:
    • pH 6.1 to 6.4
    • Osmolality 355 to 385 mOsm/kg
  6. Sterile filter into desired container using a 0.2 μm sterile filter.