Wiki Category: Biochemistry

SDSPAGE gel       4 x Sample buffer (100 ml) Name 0.5 M Tris-HCl (pH6.8) 0.25 M SDS 8 g Sucrose…

For Biochemistry Name Stock conc. Working conc. DTT (dithiothreitol) 1 M 1-10 M Protease inhibitor cocktail 10…

Protein Purification Protocol for MBP tagged proteins:   Preparation: Prepare Lysis Buffer: 50 mL per cel…