BEVS media list Company Cat. No. Name List Price (yen) Actual price (with tax) (yen) Expression systems 96-001…
Wiki Category: Molecular Biology Protocols
Bacteria culture reagent list (Antibiotics, IPTG, X-gal etc)
Antibiotics Name Stock conc. Working conc. Ampicillin 100 mg/ml 100 μg/ml Carbenicillin 100 mg/ml 100 μg/ml Ka…
E.coli media recipe
LB media Name amount (for 1 L) Bacto Tryptone 10 g Bacto Yeast Extract 5 g NaCl 5 g 5N NaOH 0.5 ml Supe…
Competent cell list
Cloning DH5 alpha KRX Protein expression BL21(DE3) B834(DE3) Rosetta (DE3) C41 C43 Baculovirus production DH1…
Making Competent cell (Rubidium chloride method)
Making Competent Cells: Grow 50mL overnight culture Inoculate 1L/500mL LB with 20mL/10mL of the O/N culture Gr…